NIPPON Genetics EUROPE provides cutting-edge products for the life science and biotechnology sector.
Nucleic Acid Extraction | Staining | Restriction Enzymes | Gel Analysis – Our product portfolio focuses on molecular biology, such as enzymes, kits and ready mixes for RT, PCR, qPCR, DNA Electrophoresis with safe DNA dyes and consumables, Protein electrophoresis with precast PAGE gels, protein marker and stains, as well as nucleic acid purification kits for DNA and RNA, and Gel documentation systems with CCD-cameras or CMOS-sensor using our unique safe blue/green LED light
MIDORI Green Xtra
- Applications: Staining of DNA/RNA in agarose gels.
- Sensitivity: Ultra-sensitive. Much better than ethidium bromide.
- Safe: Non-toxic, non-mutagenic and non-carcinogenic.
- Compatibility: Optimal for Blue/Green LED technology and Blue LED light. For UV light we recommended MIDORIGreen Advance.
- High Efficiency: Almost no background.
- Flexible: In-gel staining and post staining.
Bambanker
- Enables long term cell storage at -80°C or -196°C
- Improved cell recovery and viability after thawing
- Ready-to-use freezing media – no programmed or sequential freezing required
- Serum-free – no risk of contamination
- Usable for all know cell lines
- Long shelf life
Restriction Enzymes
Restriction enzmyes recognize short DNA sequences and cleaves double-stranded DNA at or near a specific recognition site. Restriction enzmyes are classified into four types, based on their subunit structure, cofactor requirements and specificity of cleavage.
Brochures
(q)PCR ; RT-PCR; NGS
Cloning
What are Restriction Enzymes?
Restriction enzmyes recognize short DNA sequences and cleaves double-stranded DNA at or near a specific recognition site. Restriction enzmyes are classified into four types, based on their subunit structure, cofactor requirements and specificity of cleavage.
3,000 different restriction enzymes have been discovered, which recognize over 230 distinct DNA sequences. These enzymes are routinely used for DNA modification around the world and are an indispensable tool in molecular cloning.
Different Types of restriction enzymes
Based on the structure, cofactor requirements and specificity of cleavage there are four types of restriction enzymes (Types I, II, III, and IV).
Type I restriction enzymes cleaves the DNA at a random location far away from the recognition sequence. These enzymes require both ATP and S-adenosyl-L-methionine to function.
Type II restriction enzymes cleaves the DNA within or near the recognition sequence. These enzymes do not require ATP and are independent from methylase. The type II enzymes are the most useful restriction endonucleases for the daily laboratory work. All our NIPPON Genetics EUROPE restriction enzymes are from type II.
Type III restriction enzymes cleaves DNA about 20 – 25 base pairs away from the recognition sequence. They require both ATP and S-adenosyl-L-methionine to function.
Type IV restriction enzymes cleaves only modified, typically methylated DNA in contrast to the types I-III, which are usually inhibited by methylation.
Nucleic Acid Purification
Electrophoresis (DNA/RNA)
Electrophoresis (Protein)
Gel Documentation
Lab Instruments
Lab Plastic
