New England Biolabs

Your Trusted Partner in Molecular Biology and Beyond

Restriction Endonucleases

The Leader in the Discovery and Production of Restriction Enzymes

New England Biolabs offers over 210 restriction enzymes that are 100% active in a single buffer, the rCutSmart™ Buffer. Additionally, more than 180 of these enzymes are Time-Saver qualified, allowing for DNA digestion in just 5-15 minutes or safely overnight. With a selection of over 265 restriction enzymes, NEB provides the largest commercially available range for your research needs.

PCR, qPCR & Amplification Technologies

Enhance Your PCR with Precision: NEB’s High-Fidelity Polymerases and Advanced Amplification Kits

NEB provides high-quality DNA and RNA polymerases, nucleotide mixes, kits, and master mixes, offering innovative tools for DNA amplification and PCR.

Luna® qPCR and RT-qPCR Reagents provide rapid, sensitive, and precise detection of RNA, DNA, and cDNA targets, while Q5® High-Fidelity DNA Polymerase ensures ultra-high-fidelity PCR performance. OneTaq® offers reliable results for routine PCR, and Phusion® delivers high-fidelity PCR for more demanding applications.

DNA Modifying Enzymes

Revolutionizing DNA Research with NEB's Cloning and Manipulation Tools

NEB provides tools for DNA manipulation, including ligases, nucleases, methyltransferases, and recombinases. NEBcloner helps select products and view cloning protocols.

Next Generation Sequencing Library Preparation

High-Quality, High-Yield Libraries for a Range of NGS Applications

The success of Next Generation Sequencing (NGS) relies heavily on selecting the right library preparation workflow. High-quality libraries are crucial for various applications, including single-cell, cfDNA, and PCR-free whole-genome analysis, while sufficient yield is needed to accommodate diverse throughputs. As liquid-handling robots become more prevalent, automation compatibility has also become an increasingly important factor in optimizing the NGS process.

Markers & Ladders

NEB’s selection of DNA, RNA and protein markers and ladders combines high quality with exceptional value. DNA, RNA and protein markers are available in a range of sizes and in convenient unstained or prestained formats.

Nucleic Acid Purification

Monarch: The Ultimate Solution for High-Purity DNA Extraction

Monarch nucleic acid purification kits provide fast and reliable purification of high-quality DNA from bacterial cultures, agarose gels, and enzymatic reactions using best-in-class silica column technology.

RNA Synthesis and Modification

The Fast and Efficient Solution for High-Throughput PCR Cloning

RNA plays crucial roles in genetic information transfer, catalysis, and gene regulation, with new classes of non-coding RNAs recently discovered. NEB offers RNA reagents in bulk, GMP-grade*, or custom formats for advanced research and development.

DNA Assembly, Cloning and Mutagenesis Kits

Leading Solutions for DNA Assembly, Efficient PCR Cloning, and Rapid Site-Specific Mutagenesis with NEB Kits.

New England Biolabs offers a range of solutions for DNA assembly, including NEB Gibson Assembly®, NEBuilder® HiFi DNA Assembly (with higher fidelity polymerase), and Golden Gate Assembly for cloning and synthetic biology. For PCR cloning, NEB’s PCR Cloning Kit provides an affordable, rapid alternative to TOPO® cloning, enabling quick and simple cloning without purification, with a unique mechanism for background colony suppression. Additionally, the Q5 Site-Directed Mutagenesis Kit allows for rapid, site-specific mutagenesis of plasmid DNA in under 2 hours, offering an efficient alternative to QuikChange™. Both PCR cloning and mutagenesis kits are available with or without competent cells.

Genome Editing

NEB Simplifies CRISPR/Cas9 Editing with Advanced Reagents and Tools

New England Biolabs (NEB) provides reagents for efficient CRISPR/Cas9 genome editing, including plasmid-based delivery of Cas9 and sgRNA, direct introduction of Cas9 RNP, and tools for edit detection via NGS or enzymatic assays, streamlining workflows.

  • EnGen Spy Cas9 HF1: for high-fidelity genome engineering by direct introduction of active nuclease complexes with reduced off-target cleavage
  • EnGen® Spy Cas9 NLS, EnGen Lba Cas12a, EnGen Sau Cas9, EnGen Seq1 Cas9: for genome engineering by direct introduction of active nuclease complexes
  • EnGen® sgRNA Synthesis Kit: for rapid generation of microgram quantities of custom sgRNA
  • EnGen® Mutation Detection Kit: for robust determination of targeting efficiency
  • EnGen® Spy Cas9 Nickase: programmable site-specific DNA nicking for genome editing and in vitro applications
  • EnGen Spy dCas9 (SNAP-tag): Programmable DNA binding activity compatible with broad variety of SNAP-tag conjugates
Mutation Detection Reagents and Kits
Programmable Nucleases
sgRNA Synthesis Reagents and Kits

Cellular Analysis

Advanced Protein Labeling for Multiple Applications with SNAP- and CLIP-tags

SNAP- and CLIP-tag systems enable covalent attachment of various molecules to proteins. The process involves cloning the protein as a SNAP-tag fusion and labeling with a chosen substrate. SNAP-tag uses a benzyl linker to attach substrates like dyes, biotin, or beads. CLIP-tag allows orthogonal, simultaneous labeling of two proteins in the same cells.

Epigenetics

NEB Revolutionizes Epigenetic Analysis with Innovative Tools

Epigenetics studies heritable gene expression changes not encoded in DNA, crucial in development and disease. Advanced tools to decode the epigenome and epitranscriptome aid research, diagnostics, and therapeutics. NEB leverages enzymology expertise to provide innovative solutions for epigenetic analysis

Protein Expression

NEB Sets the Standard in Protein Expression with NEBExpress® Solutions

New England Biolabs (NEB) offers advanced tools for protein expression, including the NEBExpress® product line, designed for high-level expression and purification. Backed by 40+ years of expertise, all products are rigorously tested for quality and performance. NEB ensures reliable solutions for both research and manufacturing needs.

Protein Purification

Advanced Protein Purification Tools with NEBExpress™ Portfolio

New England Biolabs offers advanced protein purification tools through the NEBExpress™ portfolio, backed by over 40 years of recombinant protein technology expertise. Our solutions are rigorously tested for optimal performance. Trusted by researchers and used in our own processes.

Competent Cells

NEB Provides High-Performance Cloning and Protein Expression Strains for E. coli and Yeast

Cloning Strains: For cloning experiments choose from several high efficiency competent cell strains. These E. coli strains are T1 phage resistant and are endA deficient for high quality plasmid preparations. Additionally, all competent cells from NEB are free of animal products.

Yeast: NEB offers chemically competent Kluyveromyces lactis cells and variants of this strain that have been tailored for specific protein expression needs. These cells are suitable for transformation with any of our linearized pKLAC series expression vectors..

E. coli for Protein Expression: NEB offers several strains with varying levels of expression control, each with T1 phage resistance and extremely high transformation efficiencies.

Protein Tools

New England Biolabs offers proteases, phosphatases, kinases, chitinases and protein markers and ladders, as well as tools for phage display and protein labeling.

Glycobiology

New England Biolabs provides a range of endo- and exoglycosidases for modifying and removing glycans from glycoproteins in glycobiology studies. Additionally, we offer heparin lyases (heparinases) for the digestion of heparin and heparan sulfate.

DNA Plasmids

NEB offers a selection of common and specialized DNA plasmids for use in cloning experiments and applications such as protein expression, gene expression, and cellular analysis.

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