Custom Double Dye & MGB Probe Design
Eurogentec is a supplier of Double-Dye Oligonucleotide probes and offers the largest number of fluorescent reporters and quenchers. Double-Dye Oligonucleotide probes with TAMRA or various non-fluorescent quenchers (e.g. the Black Hole Quencher™ family) and a variety of fluorescent dyes can be conveniently ordered using our web ordering interface.
Furthermore, as many applications, like genotyping or gene expression profiling, are requiring small amounts of several different probes, Eurogentec has developed a high-throughput platform to produce high quality small scale Real-Time qPCR Double-Dye probes. All Double-Dye Oligonucleotides are HPLC purified. They are controlled by analytical HPLC and MALDI-TOF Mass Spectrometry. Double-Dye Oligonucleotides are provided lyophilized in individual tubes. The optimal recommended length of Double-Dye Oligonucleotide probes is 25 bases. The maximum length of Double-Dye Oligonucleotides is 35 bases. In case of extra-long Double-Dye Oligonucleotides probes are required, quenchers like Black Hole Quencher™, Deep Dark Quencher I and TAMRA can be coupled internally.
By compiling the most documented strategies for the design of effective siRNA sequences, Eurogentec has co-developed an exclusive siRNA design platform. PhD-level scientists use this reliable interface to design custom siRNA for any target of your choice.
Based on the most documented strategies for the design of effective siRNA sequences, Eurogentec has co-developed an exclusive siRNA design platform. PhD-level scientists use this reliable interface to design custom siRNA for any target of your choice.
Our free design service allows each customer to get 5 free siRNA design. For each partial or complete CDS provided, you get three siRNA candidate sequences by e-mail. To date, hundreds of customers have requested this free design service to benefit from a guaranted silencing*.
In just four easy steps, you can take advantage of our free siRNA design service:
- Complete the siRNA Email Design Request Form and submit it to our Customer Help Desk.
- After evaluating your request, we will design and send 3 siRNA candidate sequences by e-mail.
- Review the design results and order from one to all three of the siRNA sequences.
*Guaranteed Silencing :
– We guarantee up to 80 % silencing of your gene of interest with at least 1 of the 3 duplexes designed and synthesized by Eurogentec.
Custom Ab Development
Whether you are a research laboratory, a university centre or a pharmaceutical company, Eurogentec offers you a range of flexible antibody services tailored to your specific needs. Immunisation in diverse hosts can be performed with several antigens such as peptides, proteins, cell extracts or DNA. These antigens can be provided by you or produced by Eurogentec.
We are experts in the generation of polyclonal (pAb) and monoclonal (mAb) antibodies for simple projects to large and high-demanding projects.
We offer a continuum of production capabilities including manufacturing for therapeutic applications. We can produce your recombinant Ab fragment (including Fab, scFv and nanobody) in our FDA inspected GMP Biomanufacturing facility for use in human clinical trials.
Custom MonoclonalsmAb in vitro productionBoost hybridoma productivityHybridoma’s development
Antibody fragmentsCamelid polyclonals and VHHGMP Ab fragments
Solutions for IndustryCustom Polyclonal AntibodiesHybridoma developmentin vitro mAb productionVHH fragmentsGLP analytical & animal studiesAntigen design & production
Additional informationEpitope mappingAdditional servicesHow to provide my antigensStorage conditionsAnimal welfare
miRNA mimics & inhibitors
Custom Peptide Synthesis
Since our inception in 1993, AnaSpec has assisted scientists in the design and synthesis of >50,000 peptides, with many resulting in high-impact citations (featured list). Recognized for our high quality standards and technical service, we have the knowledge and expertise to offer optimal custom peptide solutions to our customers. AnaSpec routinely completes difficult to synthesize peptides, such as synthesis of peptides containing Unusual Modifications (Acetylation, Biotinylation, Citrullination, Glycosylation, Methylation, Phosphorylation, Pegylation, Small molecule conjugations), Cyclic peptides, FRET peptides, peptides for Quantitative Proteomics and many more Modifications (more about our custom peptide services). AnaSpec also offers GMP peptides for specialized applications.
Histone ChIP-seq Services
Use our services to explore ChIP-seq histone modifications down to 10,000 cells
Post-translational modification of histones regulates gene expression. With our Epigenomic Profiling Services, study regulatory elements and their interacting proteins with active promoter and enhancer analysis. Profile genome-wide histone modifications by ChIP-seq analysis to understand transcriptional regulation.
Learn more about:
- Active promoters: H3K4me3 enrichment
- Inactive promoters: H3K27me3 enrichment
- Enhancers: H3K4me1 and H3K27ac enrichment in regulatory regions
- Active gene bodies: H3K36me3
Transcription factor ChIP-seq Services
Explore the effects of transcription factor binding through ChIP-seq analysis of a multitude of TFs including:
- CTCF: transcriptional repressor and insulator activity
- p300/CBP: histone acetyltransferase
- Pol II, p53, and more
- Epigenetic writers, readers, erasers
- Nuclear receptors
- Tumorsupressor genes
Epigenomics Profiling Services
Assessing the genome-wide chromatin accessibility from individual cells provides unique insights of gene expression regulation for a number of fields such as…
ATAC-seq service (Assay for Transposase-Accessible Chromatin)
ATAC-seq (Assay for Transposase-Accessible Chromatin using sequencing) allows for assessing genome-wide chromatin accessibility. The technology is based on t…
Bioinformatics Data Mining Service
New! Data mining using machine learning (AI) for unique epigenetic data insights WHITE PAPERS Smokers vs non-smokers Breast cancer…
ChIP-seq/ChIP-qPCR Profiling service
What we give to you – ChIP-sequencing and ChIP-qPCR analysis Integrative or individual analyses for chromatin with ENCODE standards End-to-end wet lab se…
DNA Methylation Profiling (RRBS Service)
*Offer valid only until March 31, 2020 for a minimum of 10 samples. Samples must be provided in increments of 10. Alignment to reference genome a…
Illumina Infinium MethylationEPIC array BeadChip (850K) service
The Infinium MethylationEPIC Array is a genome-wide DNA methylation analysis technique based on bisulfite conversion and Illumina® technology. It allow…
MeDIP-seq service (Methylated DNA IP Sequencing)
Methylated DNA immunoprecipitation sequencing (MeDIP-Seq) is used to study 5-mC genome-wide. In this assay, specific antibodies against 5-mC are used to capt…
RNA sequencing uses next-generation sequencing (NGS) to uncover the presence and quantity of RNA for gene expression profiling across the transcriptome. Diag…
- Small RNA and miRNA
- Messenger RNA (mRNA)
- Whole transcriptome analysis and long non-coding RNA (lncRNA) analysis
Targeted DNA methylation service
Targeted DNA methylation analysis allows for the quantitative study of cytosine methylation at known and precise locations throughout the genome.
Chromatin | DNA Methylation | RNA-seq Analysis
Our Epigenomics Profiling Services assure the sample preparation expertise and quality data that you seek. We provide epigenome-wide analyses for understanding epigenetic mechanisms, epigenetics-related drug discovery, transgenerational epigenetics studies, epigenetic biomarker identification (including epigenomic cancer biomarkers), and functional epigenomics. Diagenode offers expert epigenomics services that you can trust for chromatin, DNA, and RNA analysis.
Chromatin analysis
- ChIP-seq analysis Histone modification
- Promoter analysis
- Enhancer analysis
- ChIP-seq analysis Transcription factor
- (sc)ATAC-seq Chromatin accessibility
- ChIP-seq Cas9 off-target
- Customized NGS service
DNA methylation analysis
- Reduced representation bisulfite sequencing (RRBS)
- Whole genome bisulfite sequencing analysis (WGBS)
- Targeted methylation
- MeDIP-seq/hMeDIP-seq
- Illumina Infinium MethylationEPIC array BeadChip (850K)
RNA-seq analysis
- Small RNA-sequencing
- mRNA analysis
- Whole Transcription analysis
- Gene expression profiling
Software and Image Analysis from ibidi