ATAC-seq Kit, Assay for Transposase-Accessible Chromatin : an acronym for Assay for Transposase-Accessible Chromatin followed by next-generation sequencing, stands as a pivotal technology revolutionizing the comprehensive mapping of accessible chromatin within the genome.
This innovative approach hinges upon the utilization of the transposase Tn5, a molecular tool adept at cleaving exposed open chromatin regions while concurrently affixing adapters for subsequent amplification and sequencing.
The multifaceted nature of ATAC-seq methodology presents a spectrum of capabilities:
ATAC-seq Kit, Assay for Transposase-Accessible Chromatin
Insight into Gene Regulation and Open Chromatin Signatures: ATAC-seq enables a deeper comprehension of gene regulation mechanisms by unraveling the distinctive signatures within open chromatin landscapes. These signatures serve as critical indicators of active regulatory regions within the genome.
Nucleosome Position Determination at Single-Nucleotide Resolution: With unparalleled precision, ATAC-seq facilitates the determination of nucleosome positions at a resolution of single nucleotides, providing detailed insights into chromatin structure and organization.
Revelation of Transcription Factor (TF) Occupancy: By discerning TF occupancy, ATAC-seq allows for the identification and characterization of binding sites for transcription factors, shedding light on crucial aspects of gene expression regulation.
Diagenode’s ATAC-seq kit is founded upon a rigorously validated protocol meticulously optimized for processing 50,000 cells per reaction.
The kit comprehensively comprises essential reagents necessary for seamless execution across various experimental replicates. Key features of the ATAC-seq kit include.
ATAC-seq kit features
A minimal requirement of 50,000 cells per reaction ensures efficient utilization of the kit while maintaining high-quality outputs.
The protocol boasts robustness and exhibits high reproducibility, ensuring consistency between experimental replicates and across recurring experiments.
Facilitates easy and efficient DNA capture post-tagmentation reaction through the utilization of Diagenode’s MicroChIP DiaPure columns, an integral inclusion within the kit.
Integration of a supplementary qPCR step aids in determining the optimal number of cycles required for library amplification. This crucial step serves various purposes:
Prevents Over-Amplification: By determining the precise number of cycles needed, the risk of over-amplification is mitigated, preserving the integrity and fidelity of the obtained data.
Adaptability for Challenging Samples: Offers adaptability and flexibility for more challenging sample types, enhancing the probability of successful library preparation.
Early Detection of Experimental Issues: Serves as an early indicator if the experimental procedure encounters obstacles, such as the absence of qPCR amplification, thereby enabling prompt troubleshooting and optimization.
In addition to the aforementioned features, it’s important to note that while the ATAC-seq kit from Diagenode includes the necessary reagents for cell lysis, nuclei extraction, tagmentation, DNA purification, and library amplification, the primer indexes essential for multiplexing are not provided within the kit and need to be procured separately.
