Protein Biology – Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science, with annual revenue exceeding $25 billion. Our Mission is to enable our customers to make the world healthier, cleaner and safer. Whether our customers are accelerating life sciences research, solving complex analytical challenges, improving patient diagnostics and therapies or increasing productivity in their laboratories, we are here to support them.
Protien Ladder
Protein standards and ladders for SDS-PAGE, western blots, and Isoelectric focusing (IEF) Choose from a variety of protein standards and ladders (molecular weight markers) for protein electrophoresis applications. Each protein standard or ladder is supplied in a ready-to-use format, eliminating the need to reduce, pre-mix, or add loading dyes. These protein standards and ladders are consistent from lot to lot and are strictly quality controlled on appropriate gels to help ensure consistent band migration and intensity. All prestained protein ladders are compatible for use in western blotting.
Pierce™ ECL Western Blotting Substrate
- Sensitivity: low picogram
- Stability: 1-hr working solution stability, 1-year kit stability at 4°C
- Compatibility: nitrocellulose and PVDF membranes
- Signal duration: 1 to 2 hours
- Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–10 µg/mL)
- Recommended secondary antibody concentration: 1:1,000–1:15,000 dilution (0.07–1.0 µg/mL)
Pierce™ BCA Protein Assay Kit
- Colorimetric—estimate visually or measure with a standard spectrophotometer or plate reader at 562 nm
- Uniformity—exhibits less protein-to-protein variation than dye-binding methods (Bradford)
- Compatibility—unaffected by typical concentrations of most ionic and non-ionic detergents
- Assay time—30-min incubation; much easier and four times faster than classical Lowry methods
- Assay range—linear working range for BSA of 20 to 2000 µg/mL
- Sensitivity—detect down to 5 µg/mL with enhanced protocol
Brochures
Antibodies
Thousands of Invitrogen primary and secondary antibodies validated and cited for flow cytometry, IHC/IF/ICC, western blotting, ELISA and other applications.
Western Blotting
Western blotting is an invaluable tool for protein biology. Find the right set of products that can assist you with detection and analysis of your protein. Find a comprehensive suite of solutions for every step of the western blotting workflow in order to help you obtain high-quality, publishable results with minimal time and effort.
Protein Assays
Accurately quantifying total protein concentration is a key step in most experiments and workflows involving isolation, separation, and analysis of proteins by biochemical methods. The choice among available protein assays typically is based upon several factors, including its chemical compatibility with buffer components of the samples to be assayed. We offer a variety of assay reagents, kits and standards for protein quantitation by fluorescent or colorimetric detection with fluorometers, spectrophotometers, and plate readers.
Immunoprecipitation
Immunoprecipitation magnetic beads is the fastest growing method for ip and that Invitrogen Dynabeads products are the most used and referenced products in this trend. Why? Because Dynabeads magnetic beads offer you the best balance of capacity/yield, reproducibility, purity, and cost for smaller-scale isolation of specific proteins (e.g., immunoprecipitation, IP) and protein complexes (co-immunoprecipitation, co-IP).
Protein Purification
Find the best tools for your workflow from our broad selection of resins and formats for the purification of proteins and antibodies, or create a customized purification solution by covalent attachment of a ligand to one of our activated supports.
Our prepared and activated magnetic beads, magnetic agarose beads, standard and Superflow agarose resins, Applied Biosystem POROS resins, and other chromatography media enable protein purification at nearly any scale: assay, screening, batch, pilot, or process. In addition, we’ve developed a range of accessory products for all aspects of purification, including disposable columns and binding and elution buffers including disposable columns, binding and elution buffers, and endotoxin quantitation and removal products.
Cell Lysis and Protein Extraction
Cell lysis is the first step in cell fractionation, organelle isolation and protein extraction and purification. As such, cell lysis opens the door to a myriad of proteomics research methods. Many techniques have been developed and used to obtain the best possible yield and purity for different species of organisms, sample types (cells or tissue) and target molecule or subcellular structure.
Dialysis and Desalting
After cell lysis or isolation, lysates often contain contaminants that are not compatible with downstream steps. We offer a variety of specialty devices and resins that simply and efficiently desalt, exchange buffer, and remove detergents from samples. In addition, if the protein concentration is too dilute for further processing or analysis, the sample can be concentrated quickly using centrifugal concentrators.
Protein Labeling and Crosslinking
We offer a wide range of products for the crosslinking, biotinylation, pegylation and fluorescent labeling of proteins, antibodies and peptides. These bioconjugation reagents are commonly used to studying the structure and interaction of proteins, build immunoassays, increase protein solubility or attach molecules to surfaces. Use our crosslinking or biotinylation reagent selection tool to find the optimal reagent for your application, or access our helpful technical handbook to help improve your bioconjugation result.
Mass Spec Analysis
We offer a wide range of products for protein mass spectrometry. Our discovery quantitation reagents and kits enable higher multiplexing using SILAC NeuCode technology or TMT11plex isobaric labeling. Achieve relative or absolute quantitation for targeted applications by using SureQuant Targeted MS assays or Heavypeptide AQUA custom peptides. Our sample preparation products enable efficient abundant protein depletion, immunoprecipitation, protein digestion, phospho-peptide enrichment, and peptide clean-up. Our calibration solutions, standards and solvents are optimized for instrument calibration and QC. Use our interactive workflow guide or access our technical resources to find the best product for your application.
Protein Gel Electrophoresis
Protein gel electrophoresis is a simple way to separate proteins prior to downstream detection or analysis. Our portfolio of high-quality protein electrophoresis products unites gels, gel tanks, protein gel handcast system, stains, molecular weight markers and standards, running buffers, and blotting products for your protein analysis experiments. Find a protein electrophoresis workflow that matches your separation task using the products below.
