At Diagenode, our goal is to build products with pride and the highest level of performance.
The company offers innovative Boruptor shearing and IP Stare automation instruments, reagent kits, and high quality antibodies to streamline DNA methylation Chip, and ChIP-seq workflows. The company’s latest innovations include a unique, full automation system, the industry’s most validated antibodies, the Megaruptor shearing system for long fragment generation in sequencing, and epigenetics assay services
Brochures
Chromatin immunoprecipitation (ChIP)
Chromatin immunoprecipitation (ChIP) is a method used to determine the location of genome binding sites for a specific protein of interest, giving invaluable insights into the regulation of gene expression. ChIP involved the selective enrichment of a chromatin fraction containing a specific antigen. Antibodies that recognize a specific protein or protein modification are used to determine the relative abundance of the antigen at specific loci. ChIP-seq and ChIP-qPCR are techniques that allow the identification of protein-DNA binding sites.
DNA Methylation
Understanding DNA methylation is critical for learning about control of transcription, genomic imprinting, embryonic development, and carcinogenesis. Diagenode proposes two main approaches to studying DNA methylation – bisulfite conversion with downstream sequencing or qPCR and immunoprecipitation (IP).
Shearing Technologies
Diagenode focuses on state-of-the-art preparation of high quality biological and chemical samples by developing the industry’s most advanced water bath sonicators and hydrodynamic devices. Our instruments are ideal for a number of applications in various fields of studies including environmental research, toxicology, genomics and epigenomics, cancer research, stem cells and development, neuroscience, clinical applications, agriculture, and many more.
Antibodies
Since 2004 Diagenode has been the most trusted supplier for epigenetics-focused antibodies. We understand that high quality is crucial for the success of your ChIP and ChIP-seq experiments, so we emphasize strict quality standards and actual validation for all of our antibodies. Our partnerships with leading epigenetics experts and epigenetics consortiums such as BLUEPRINT have allowed us to validate our antibodies to the highest degree, guaranteeing specificity, reproducibility, and success for each and every ChIP assay. In addition, unlike competing suppliers, we are fully transparent with our validation and provide all batch-specific validation data for tested applications on every data sheet right here on our website.
NGS Library Preparation
Diagenode provides a complete portfolio of library preparation kits for Next-Generation Sequencing, covering RNA-seq, ChIP-seq, and DNA sequencing. Our library preparation solutions can be used for low inputs, from as little as 10 pg of starting material. Our kits offer simple and fast workflows, high yields, and ready-to-sequence DNA. Our solutions incorporate user-friendly protocols that even beginners can easily master upon the first use.
Automation
Diagenode offers a variety of end-to-end systems to streamline DNA methylation and chromatin immunoprecipitation workflows. Diagenode’s IP-Star® Compact is a simple, automated bench-top instrument that standardizes different epigenetics applications (i.e. ChIP, MeDIP or NGS Library preparation) and library preparation. The platform offers full workflow support for epigenetics research, utilizing our complete kits and laboratorlidated protocols to rapidly deliver high-quality and consistent data.
