jetPEI- In vivo delivery of DNA, siRNA, shRNA and miRNA
In the ever-evolving landscape of molecular biology and genetic research, the choice of a transfection reagent can significantly impact the success of experiments. Among the standout options in the market, jetPEI has proven to be a reliable ally for scientists, offering a range of capabilities that set it apart.
Tailored Protocols for Diverse Applications:
One of the key strengths of jetPEI lies in its adaptability, thanks to the availability of three distinct protocols catering to various experimental needs.
Forward Protocol:
The forward protocol is a strategic approach where cells are split the day before transfection, and jetPEI/DNA complexes are added to either adherent or suspension cells.
This method provides a systematic and organized way of introducing genetic material into the cells, ensuring optimal conditions for successful transfection.
Reverse Protocol: jetPEI- In vivo delivery of DNA, siRNA, shRNA and miRNA
For experiments involving a pool of genes, such as those utilizing a DNA library, the reverse protocol proves to be the most fitting. In this scenario, jetPEI/DNA complexes are prepared or deposited in the wells before the addition of cells. Remarkably, these complexes remain stable for up to 4 hours, providing researchers with a generous window of time to carry out their experiments.
Batch Protocol:
The batch protocol, on the other hand, is a time-saving option designed to prepare a homogeneous pool of transfected cells. Particularly useful for drug screening applications, this protocol involves transfecting cells just after trypsinization while still in suspension.
Notably, this approach allows for a rapid processing time, providing results a day earlier than the forward protocol.
Robust Transfection Complexes for Reliable HTS:
The robustness of jetPEI transfection complexes is a testament to its efficiency in High-Throughput Screening (HTS).
The complexes, formed with the water-soluble polymer jetPEI and DNA, remain stable for up to 4 hours. This is a significant advantage over lipid-based reagents and calcium phosphate, providing researchers with ample time to dispense the complexes into the plates without compromising their efficacy.
Efficiency Across a Wide Range of Cell Types:
A standout feature of jetPEI is its ability to efficiently deliver genes to a diverse array of cell types. This includes adherent and non-adherent cell lines, as well as primary cells.
The extensive validation is reflected in over 550 publications found in the Polyplus-transfection Database, demonstrating the widespread use and trust in jetPEI across the scientific community.
