PULSin – Best Protein, antibody and peptide delivery reagent 2024
PULSin® is a protein, antibody, and peptide delivery reagent developed by Polyplus Transfection. It is a powerful and innovative tool that enables the delivery of proteins and antibodies into mammalian cells without the need for chemical conjugation or viral transduction.
PULSin® is designed for the delivery of proteins, antibodies, and peptides into cells. It is a transfection reagent specifically optimized for the delivery of these molecules without the need for transfection agents that might interfere with their functionality.
PULSin® is composed of a proprietary cationic amphiphile molecule that forms non-covalent complexes with proteins and antibodies. Once formed, these complexes are internalized via anionic cell-adhesion receptors and are released into the cytoplasm where they disassemble. The process is non-toxic and delivers functional proteins.
PULSin® is optimized for efficient delivery in live primary cells and difficult-to-transfect cells. It is also an easy-to-use alternative to chemical conjugation and viral transduction.
PULSin® enables delivery of protein irrespective of its size
Punsin showcases impressive capabilities in transporting R-phycoerythrin—a fluorescent protein with a considerable size of 240 kD—into the cytoplasm of cells with remarkable efficiency, reaching up to 98% of cells.
Upon successful delivery, this protein undergoes even dispersion within the cytoplasmic space.
Notably, owing to its substantial molecular size, R-phycoerythrin is effectively prevented from entering the cell nucleus, resulting in its exclusion from this compartment.
This selective distribution within the cytoplasm without nuclear entry highlights the precision and efficacy of PULSin® in facilitating the intracellular delivery of large proteins, offering a promising avenue for various cellular and molecular studies as well as potential therapeutic applications.
Pulsin Performance in Antibody Delivery
Protocol For Protein Delivery
Utilizing PULSin® presents a significant advantage in terms of time and effort when compared to alternative methods such as viral transduction or chemical conjugation.
This ready-to-use reagent arrives conveniently equipped with a dilution buffer and a fluorescent control protein (R-phycoerythrin), streamlining the process for immediate application.
The protocol is notably swift: the procedure involves simply adding the reagent to the protein, incubating the mixture, and subsequently introducing it to the cells.
Post-delivery, cells can be analyzed in as little as 4 hours, allowing for rapid assessment and study of the protein’s effects within the cellular environment.
This streamlined approach minimizes preparation time and offers an expedited pathway for researchers to delve into cellular analyses and investigations without protracted waiting periods.
Alternative to Chemical Conjugation and Viral Transduction
The evaluation of PULSin® against two alternative protein delivery reagents reveals superior efficiency in delivering proteins. Furthermore, the quantity of protein delivered per cell is notably amplified when using PULSin®. This enhanced delivery capacity is evident in both the R-phycoerythrin protein and the FITC-alpha-tubulin antibody, as confirmed by supplementary data.
Read related articles here!!
